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. 2020 Mar 1;47(1):42–53. doi: 10.5653/cerm.2019.03013

Figure 3.

Figure 3.

Experimental validation of predicted microRNAs (miRNAs) targets using luciferase assays. Luciferase assays were performed to validate the interaction of miRNAs with their target genes. Transient overexpression of miRNAs, including miR-371a-5p (A), miR-3065-3p (B), miR-519b-3p (C), and miR-373-3p (D), reduced the luciferase activity of 3' untranslated region reporters of their target genes (LEF1, LOX, ITGB4, and CD44, respectively). Values represent mean ± standard error. NC, negative control. a)p< 0.05.