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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Cancer Res. 2020 Feb 10;80(7):1401–1413. doi: 10.1158/0008-5472.CAN-19-3094

Figure 3. DUOX1 and DUOX2 mRNA expression is decreased in PDAC cell lines compared to ductal epithelium in vitro and is increased in a dose-dependent manner after exposure to P-AscH−.

Figure 3.

MIA PaCa-2, PANC-1 and H6c7 ductal epithelial cells were exposed to varying doses of P-AscH− for 1 h. mRNA expression was tested 24 h after exposure to P-AscH−. For figures A-B and E-J: Means ± SEM, ANOVA with Tukey’s multiple comparisons used.

A-B. DUOX1 and DUOX2 mRNA expression is decreased in PDAC cell lines MIA PaCa-2, PANC-1, 339, and 403 as compared to non-tumorigenic H6c7 cells (n = 3, *p < 0.05 vs. H6c7).

C-D. DUOX1 and DUOX2 immunoreactive protein is decreased in PDAC cell lines as compared to non-tumorigenic H6c7 cells. Representative blots shown.

E-F. DUOX1 and DUOX2 mRNA expression is increased in MIA PaCa-2 by increasing doses of P-AscH− (n = 3–5, *p < 0.05 vs. 0 mM).

G-H. DUOX1 and DUOX2 mRNA expression is increased in PANC-1 by increasing doses of P-AscH− (n = 6–7, *p < 0.05 vs. 0 mM).

I-J. DUOX1 and DUOX2 mRNA expression is unchanged in non-tumorigenic H6c7 cells by increasing doses of P-AscH− (n = 3–4, p > 0.05 vs. 0 mM).