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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Cancer Res. 2020 Feb 10;80(7):1401–1413. doi: 10.1158/0008-5472.CAN-19-3094

Figure 4. DUOX1 and DUOX2 expression is increased in a time dependent manner after exposure to P-AscH− in PDAC cells.

Figure 4.

−MIA PaCa-2, PANC-1 and H6c7 ductal epithelial cells were exposed to P-AscH− and DUOX expression determined at 0–72 h. For figures A-B, D-E, and G-J: Means ± SEM, ANOVA with Tukey’s multiple comparisons used.

A-B. DUOX1 and DUOX2 mRNA expression is increased following P-AscH− (5 mM) in MIA PaCa-2 cells (n = 3, *p < 0.05 vs. 0 h).

C. DUOX1 and DUOX2 immunoreactive protein is increased in MIA PaCa-2 cells after P-AscH− (5 mM). Representative blots shown.

D-E. DUOX1 and DUOX2 mRNA expression is increased following P-AscH− (5 mM) in PANC-1 cells (n = 4 – 5, *p < 0.05 vs. 0 h).

F. DUOX1 and DUOX2 immunoreactive protein is increased in PANC-1 after P-AscH− (5 mM). Representative blots shown.

G-H. DUOX1 and DUOX2 mRNA expression is increased following P-AscH− (1 mM) in MIA PaCa-2 cells and is reversed by pretreatment with 100 μg/mL bovine catalase. (n = 4, *p < 0.05 vs. control).