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. 2020 Mar 24;2020:3894309. doi: 10.1155/2020/3894309

Figure 5.

Figure 5

Effect of Lipopolysaccharide (LPS) treatment on hypoxic condition in the mammary gland. Mice were injected intraperitoneally with pimonidazole HCl, and mammary tissues were treated with PBS or LPS for 12 or 24 h (n = 8). (a–c) Representative immunostaining of hydroxyprobe in the (a) PBS-treated gland and (b) LPS-treated gland at 12 h. (c) Negative control staining performed by the omission of the first antibody against hypoxyprobe. (d) Quantitative representation of relative hypoxic condition in LPS- and PBS-infused glands. mRNA expression of (e) hypoxia-inducible factor 1 (Hif1a) and (f) glucose transporter 1 (Slc2a1) by real-time PCR. Relative gene expression was calculated by the 2-ΔΔCt method and normalized by the expression levels of housekeeping genes Actb, Gapdh, Hrpt, Stx5a, and Hnrnpab. Error bars represent standard error, and different letters above each bar indicate significant differences. Significance was declared when P < 0.05.