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A multiplex PCR typing of selected Clostridium perfringens isolates. Lane 1: empty lane, all the 18 isolates showed the PCR product resulting from amplification of plc (cpa) gene showing a fragment size of 900 bp. Lanes 4-7 produced etx gene (a fragment size of (396 bp) in addition to plc gene. Lanes 12 and 14 produced cpb gene (a fragment size of 612 bp) in addition to the plc gene. Lanes 2, 3, 8-11, 13, 15-19 produced only the plc gene. Lane 20: Bioline EasyLadder 1.
