Table 1.
Primers used for amplification of the genes responsible of producing specific toxins and the expected amplicon size together with the annealing temperature for the PCR.
| Gene | Primer sequence 5′ to 3′ | Amplicon size (bp) | Annealing temp (°C) |
|---|---|---|---|
| Plc, cpa (α-toxin) | CPA5L: AGTCTACGCTTGGGATGGAA CPA5R: TTTCCTGGGTTGTCCATTTC |
900 | 55 |
|
| |||
| cpb (β-toxin) | CPBL: TCCTTTCTTGAGGGAGGATAAA CPBR: TGAACCTCCTATTTTGTATCCCA |
612 | 55 |
|
| |||
| etx (ε-toxin) | CPETXL: GGGGAACCCTCAGTAGTTTCA CPETXR: ACCAGCTGGATTTGAGTTTAATG |
396 | 55 |
|
| |||
| ipa (ι-toxin) | CPIL: AAACGCATTAAAGCTCACACC CPIR: CTGCATACCCTGGAATGGCT |
293 | 55 |
|
| |||
| Cpe (CPE) | CPEF: GGAGATGGTTGGATATTAGG CPER: GGACCAGCAGTTGTAGATA |
233 | 56 |
|
| |||
| cpb2 (β2-toxin) | CPBL: CAAGCAATTGGGGGAGTTTA CPBR: GCAGAATCAGGATTTTGACCA |
200 | 55 |
|
| |||
| NetB toxin | AKP78: GCTGGTGCTGGAATAAATGC AKP79: TCGCCATTGAGTAGTTTCCC |
560 | 55-58 |