Table 2.

Effect of inactivation treatments on Tularemia rapid test and microagglutination test

Patient	Rapid test				Microagglutination
	Control	Triton X-100 0.1%	Triton X-100 1%	Heat	Control	Triton X-100 0.1%	Triton X-100 1%	Heat
1	–	–	–	–	<10	<10	<10a	<10
2	2+	2+	2+	1+	2560	2560	1280b	1280b
3	–	–	–	–	<10	<10	<10a	<10
4	2+	2+	2+	1+	1280	1280	1280b	640b
5	2+	2+	1+	–	160	160	<10a	<10
6	2+	2+	1+	±	320	320	40a	<10
7	–	–	–	–	<10	<10	80a	<10
8	2+	2+	2+	1+	640	640	1280b	320b
9	2+	2+	2+	±	2560	2560	1280b	1280b
10	2+	2+	2+	1+	5120	5120	10240	5120b
Weighted kappa		1.000	0.851	0.353		1.000	0.657	0.684

Bold indicates false-qualitative result.

Controls and heat-inactivated samples were treated with an equivalent volume of PBS.

A negative result in the rapid test is indicated by a dash (–), whereas positive test results are indicated by ±, 1+, or 2+ (scale from weakly positive test to strongly positive test).

^aPartial agglutination.

^bProzone effect.