Table 3.
Sensitivity of direct fluorescent antibody (DFA), culture and four multiplex assays for detection and identification of respiratory viruses. Number of positives (within brackets)
| Target | DFA | Culture | Resplex II Panel v2.0 | Seeplex RV15 | xTAG® RVP | xTAG® RVP Fast |
|---|---|---|---|---|---|---|
| INFA | 76.7% (46) | 60.3% (35) | 96.9% (62) | 96.9% (62) | 98.4%a (63) | 93.7%b (60) |
| INFB | 78.4% (29) | 75.0% (21) | 100% (37) | 100% (37) | 100% (36) | 64.9% (24) |
| PIV (1–4) | 72.4% (21) | 61.5% (16) | 82.9% (34) | 97.6% (40) | 85.4% (35) | 65.8% (26) |
| PIV1 | 76.9% | 66.7% | 86.7% | 93.3% | 71.4% | 46.7% |
| PIV2 | 55.5% | 44.4% | 88.9% | 100% | 100% | 77.8% |
| PIV3 | 100% | 66.7% | 100% | 85.7% | 71.4% | 42.8% |
| PIV4 | – | – | 60.0% | 100% | 100% | 100% |
| hMPV | 68.6% (24) | 43.3% (13) | 82.0% (32) | 97.4% (38) | 97.4% (38) | 92.3% (36) |
| RSV (A/B) | 93.5% (130) | 86.5% (96) | 84.0% (121) | 100% (144) | 88.2% (127) | 91.7% (132) |
| RSVA | – | – | 90.4% | 100% | 85.5% | 92.5% |
| RSVB | – | – | 79.3% | 100% | 98.3% | 94.8% |
| ADV | 38.1% (8) | 44.4% (8) | 71.4% (15) | 100% (21) | 85.7% (18) | 52.4% (11) |
| BoV | – | – | 75.0% (18) | 100% (24) | – | 100% (24) |
| CoV OC43/HKU1 | – | – | 92.6% (25) | 100% (27) | 48.1% (13) | 59.3% (16) |
| CoV 229E/NL63 | – | – | 100% (17) | 100% (17) | 88.2% (15) | 88.2% (15) |
| Enterovirus/rhinovirus | – | – | 96.7% (172) | 71.7% (127) | 93.8% (167) | 97.7% (174) |
aBased on combination of INFA + H1 + H3 + H5.
bBased on combination of INFA + H1 + H3.