Table 5.
Performance of two commercial multiplex PCR assays vs. Astra Influenza Screen and Type assay for detection of the pandemic H1N1 2009 influenza A virus and seasonal influenza A strains
| Target | Number | DFA # (% positive) | Astra influenza Screen & Type # (% positive) | Resplex II Plus Panel PREa # (% positive) | Seeplex influenza A/B Subtyping # (% positive) |
|---|---|---|---|---|---|
| Pandemic H1N1 2009 INFA | 50 | 32/46 (69.6%) | 50 (100%) | 46 (92%) | 48 (96%) |
| INFA (seasonal H1 and H3) | 25 | 14 (56%) | 25 (100%) | 25 (100%) | 25 (100%) |
| Seasonal H1N1 INFA | 13 | 4/10 (40%) | 13 (100%) | 13 (100%) | 11 (84.6%) |
| Seasonal H3N2 INFA | 12 | 10 (83.3%) | 12 (100%) | 12 (100%) | 11 (91.7%) |
| Negative | 55 | 0 | 0 | 0 | 0 |
aResplex II Plus Panel PRE assay does not differentiate INFA subtypes (except for pandemic H1N1 2009 influenza A subtype).