Table 3.
In vitro sensitivity of the near‐patient assays for influenza A and B viruses in comparison to an in‐house ELISA and influenza A virus‐specific RT‐PCR
| Diagnostic assay/manufacturer | Lowest concentration of stock virus yielding a positive result (TCID50/mL) | ||
|---|---|---|---|
| A/Wisconsin/67/05 (H3N2) | A/New Caledonia/20/99 (H1N1) | B/Jiangsu/ 10/03 | |
| Binax Now Influenza A&B/Binax Inc. | 104 | 105 | 103 |
| Influ‐A&B Respi‐Strip/Coris Bioconcept | 104 | 106 | 103 |
| Actim Influenza A&B/Medix Biochemica | 103 | 105 | 102 |
| Antigen‐ELISA/in‐house | 103 | 105 | 103 |
| RT‐PCR/in‐house | 10−3 | 10−2 | ND |
ND, not done because RT‐PCR is only specific for influenza A viruses; TCID50, 50% tissue culture infective dose.