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. 2006 Apr 21;33(6):317–322. doi: 10.1016/j.cyto.2006.03.001

Fig. 1.

Fig. 1

Inhibitory effect of GL on M2Mϕ generation stimulated with PMN-II. (A) Time course. R-Mϕ (6 × 105 cells/well, lower chamber) were cultured with PMN-II (2 × 105 cells/well, upper chamber) supplemented with SAC (0.0075%) in a dual-chamber transwell. The transwell cultures were performed in the presence (open circles) or absence (filled circles) of a 100 μg/ml dose of GL. Culture fluids harvested were assayed for CCL17, as a parameter of M2Mϕ. (B) M2Mϕ generation stimulated with PMN-II. R-Mϕ were cultured with PMN-II (0.08–2 × 105 cells/well, upper chamber) previously supplemented with SAC in a dual-chamber transwell. The transwell cultures were performed in the presence or absence of GL (100 μg/ml). Cells were harvested from the lower chamber 18 h after cultivation and recultured for 24 h without any stimulation. Culture fluids harvested were assayed for CCL17. (C) Dose–response inhibitory effects of GL. The transwell cultures were performed with various doses of GL. Culture fluids harvested were assayed for CCL17. The data are displayed as the mean CCL17 production ± S.D. and are representative of three experiments.