Fig. 2.

M2Mϕ were not generated in cultures of R-Mϕ and GL-treated PMN-II. R-Mϕ (6 × 10⁵ cells/well, lower chamber) were cultured with PMN-II (2 × 10⁵ cells/well, upper chamber) previously treated with GL (1–100 μg/ml) in dual-chamber transwells supplemented with SAC. Cells were harvested from the lower chamber 18 h after cultivation and recultured for 24 h. Culture fluids harvested were assayed for CCL17. The data are displayed as the mean ± S.D. and are representative of three experiments.