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. 2001 Apr 11;10(1):21–28. doi: 10.1016/S1074-7613(00)80003-5

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© 1999 Cell Press

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NO Eliminates a Sulfhydryl Group from 3C^pro and Nitrosylates 3C^pro

Purified 3C^pro was incubated with AP, SNAP, spermine, or spermine NONOate. (A) Analysis of the 3C^pro sulfhydryl group. Treated 3C^pro was incubated with an excess of 5,5 dithiobis(2-nitrobenzoate) (DNTB) and its absorption measured at 412 nm. n = 4 ± SD. (B) Nitrosylation of the 3C^pro active site Cys¹⁴⁷. 3C^pro was exposed to NO donors or their controls, then incubated with HgCl₂, and the amount of nitrite released was measured by the Griess reaction. n = 4 ± SD.