Table 1.
Analytical sensitivity of the PCR Ad consensus method
| Strain | Species | GE/ml |
|||
|---|---|---|---|---|---|
| ID5O/ml | Gel electrophoresis | Group-specific hybridization | Species-specific hybridization | ||
| Ad 12 | A | 102.0 | 103.5 | 104.5 | 105.5 |
| Ad 3 | B1 | 104.0 | 104.5 | 106.5 | 106.5 |
| Ad 7 | B1 | 103.2 | 106.5 | 106.5 | 107.5 |
| Ad 11 | B2 | 103.4 | 106.5 | 108.5 | 108.5 |
| Ad 1 | C | 104.9 | 104.5 | 106.5 | 106.5 |
| Ad 8 | D | 102.9 | 104.5 | 105.5 | 105.5 |
| Ad 4 | E | 103.2 | 105.5 | 105.5 | 106.5 |
| Mean | 104.0 | 105.0 | 106.35 | 106.35 | |
Serial dilutions of MRC5 cells infected with Ad prototype strains from the main species and representative of respiratory Ad infections: Ad 12 (sg A), Ad 3 and Ad 7 (sg B1), Ad 11 (sg B2), Ad 1 (sg C), Ad 8 (sg D), Ad 4 (sg E) were used to count the number of genome equivalents per ml (GE/ml) and the 50% infectious doses per ml (ID50/ml).