Table 2.
Comparison of the diagnostic efficacies of the Ad-specific PCR methods with that of virus culture or immunofluorescence in 362 nasal aspirates samples
| Diagnostic Method | PCR Ad consensus |
PCR Ad hexon |
||
|---|---|---|---|---|
| Positive | Negative | Positive | Negative | |
| Virus culture/Immunofluorescence | ||||
| Positive | 95 | 2 | 94 | 3 |
| Negative | 18a | 247 | 13 | 252 |
| Total | 362 | 362 | ||
| Sensitivity (%) | 97.9 | 96.9 | ||
| Specificity (%) | 93.2 | 95 | ||
| PPV (%) | 84 | 87.8 | ||
| NPV (%) | 99.1 | 98.8 | ||
a
Thirteen specimens were positive in both PCR Ad consensus and PCR Ad hexon but not in according to the culture/immunofluorescence method. Five additional samples were only positive according to PCR Ad consensus, these samples were re-extracted and controlled as positive.