Table 3.
Comparison of Ad identification by the species-specific PCR Adenovirus consensus®, by type-specific neutralisation in culture and by a combination of PCR and restriction endonuclease digestion
| Number of strains | Detection by IF or culturea | PCR AdV Consensus® | PCR-RE digestionb |
|---|---|---|---|
| 1 Ad species A | 1 Ad (IF) | 1 sp A | 1 Ad 31 |
| 44 Ad species B | 36 Ad 3 | 36 sp B1 | 36 Ad 3 |
| 1 Ad 3 | 1 sp B1 | 1 negative | |
| 2 Ad 7 | 2 sp B1 | 2 Ad 7 | |
| 2 Ad (IF) | 2 sp B1 | 2 Ad 3 | |
| 1 Ad 11 | 1 sp B2 | 1 sp B2 | |
| 2 negative | 2 sp B1 | 2 negative | |
| 42 Ad species C | 9 Ad 1 | 9 sp C | 9 Ad 1 |
| 13 Ad 2 | 13 sp C | 13 Ad (2.5.6) | |
| 3 Ad 5 | 3 sp C | 3 Ad (2.5.6) | |
| 1 Ad 5 | 1 sp C | 1 negative | |
| 10 Ad 6 | 10 sp C | 10 Ad (2.5.6) | |
| 3 Ad (IF) | 3 sp C | 2 Ad 1;1 Ad (2.5.6) | |
| 3 Negative | 3 sp C | 3 Ad (2.5.6) | |
| 3 Ad species E | 3 Ad (IF) | 3 sp E | 3 sp E |
| 1 Ad species F | 1 negative | 1 sp F | Negative |
| 69 Ad negative | Negative | Negative | Negative |
a
Detection by immunofluorescence (IF), or by culture followed by a type-specific neutralisation assay adapted from Hierholzer (1995).
b
PCR and restriction endonuclease digestion, adapted from Allard et al. (2001).