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LOUIS test algorithm for H.S positive colonies
| LDC | ONPG | URE | IND | Possible indentification | STEP 1 | STEP 2 | |
|---|---|---|---|---|---|---|---|
| + | − | − | − | Salmonella | Confirm by Serology (O and H antigens) | Positive | Negative serology. |
| Full biochemical ID | Discard | ||||||
| − | − | − | − | Possible LDC negative Salmonella | API rapid ID32E (4h) | Confirm with serology | Negative serology. Discard |
| − | − | + | + | Proteus spp or Morganella morganii | Discard | ||
| − | − | + | + | ||||
| − | + | − | − | Citrobacter spp | Discard |
* Discard any other combination or reactions
