Table 1.

Polymerase Chain Reaction Conditions for Viral Amplification

Virus	Cell conditions	Number of cycles
Panleukopenia	94° C × 30 seconds, 53° C × 35 seconds, 72° C × 35 seconds. 72° C × 10 minutes	45
Herpes	94° C for 60 seconds, 55° C for 60 seconds, 72 °C for 60 seconds. 72° C × 10 minutes	45
Calici	94° C × 30 seconds, 53° C × 35 seconds, 72° C × 35 seconds. 72° C × 10 minutes	40
Corona	94° C × 50 seconds, 55° C × 60 seconds, 72° C × 60 seconds. 72° C × 10 minutes	35