Table 2.
Lower detection limits of pandemic (H1N1) 2009 ELISA on pandemic (H1N1) 2009 virus cultures
| Virusa | Lower detection limit | |
|---|---|---|
| TCID50/mL (in log10) | HA titreb | |
| A/Xiamen/N465/2009(H1N1) | 2.9 | 0.008 |
| A/Xiamen/N582/2009(H1N1) | 2.8 | 0.008 |
| A/Xiamen/N584/2009(H1N1) | 2.9 | 0.004 |
| A/Xi’an/A29/2009(H1N1) | 3.4 | 0.004 |
| A/Xi’an/A35/2009(H1N1) | 2.4 | 0.016 |
| A/Xi’an/A36/2009(H1N1) | 3.6 | 0.008 |
| A/HK/41 9521/2009(H1N1) | NA | 0.008 |
| A/CA/04/2009(H1N1) | NA | 0.016 |
| Average LDL(mean ± SD) | 3.0 ± 0.4 | 0.009 ± 0.005 |
CA, California; HA, haemagglutinin; HK, Hong Kong; LDL, lower detection limit; NA, not applicable; SD, standard deviation; TCID, tissue culture infective dose.
aA/HK/41 9521/2009(H1N1) and A/CA/04/2009(H1N1) were inactivated viral cultures of allantoic fluids, and others were live viral cultures of MDCK cell supernatants.
bThe HA titre is the reciprocal of the highest dilution of virus with complete haemagglutination.