Table 2.

Temperature and time conditions for the PCRs

	h-Hp		β-Actin		PC5		Furin		Albumin
Pre-denaturation	95 °C	1 min	95 °C	1 min	95 °C	1 min	95 °C	1 min	94 °C	5 min
Denaturation	94 °C	40 s	94 °C	30 s	95 °C	30 s	95 °C	30 s	94 °C	1 min
Primer annealing	55 °C	30 s	55 °C	30 s	60 °C	30 s	60 °C	30 s	62 °C	1 min
Extension	72 °C	40 s	72 °C	1 min	72 °C	60 s	72 °C	1 min	72 °C	1 min
Number of cycles	34		30		40		40		35
Final extension	72 °C for 5 min		72 °C for 5 min		72 °C for 5 min		72 °C for 5 min		72 °C for 10 min
Cooling	4 °C		4 °C		4 °C		4 °C		4 °C

h-Hp, human haptoglobin; PC5, proprotein convertase subtilisin.