Table 4.
Details of the peptide summary reporta
| Query | Observed | Mr(expt) | Mr(calc) | Delta | Miss | Score | Expect | Rank | Peptide |
|---|---|---|---|---|---|---|---|---|---|
| P00739 (HPTR_HUMAN)b | |||||||||
| 385 | 490.12 | 978.22 | 977.50 | 0.72 | 0 | (38) | 0.027 | 1 | K.NPANPVQR.I |
| 386 | 490.15 | 978.29 | 977.50 | 0.79 | 0 | 42 | 0.012 | 1 | K.HPVDQVQR.I |
| 456 | 538.17 | 1074.33 | 1074.58 | −0.25 | 1 | 3 | 95 | 5 | R.MLCVRLGAR.N |
| 651 | 712.57 | 1423.12 | 1422.76 | 0.36 | 1 | (31) | 0.1 | 1 | K.NDVTDISDDRFPK.C |
| 652 | 475.75 | 1424.24 | 1422.76 | 1.48 | 1 | (31) | 0.098 | 1 | K.NDVTDISDDRFPK .C |
| 653 | 475.77 | 1424.29 | 1422.76 | 1.53 | 1 | 41 | 0.0091 | 1 | K.NDVTDISDDRFPK.C |
| 665 | 485.05 | 1452.14 | 1451.70 | 0.44 | 1 | 4 | 51 | 10 | K.DYVAPGRMLCVR.L + oxidation (M) |
| 784 | 834.45 | 1666.88 | 1666.80 | 0.08 | 0 | 58 | 0.00018 | 1 | K.LTLYVGKKQLVEIEK.Q |
| P09958 (FURIN_HUMAN)c | |||||||||
| 504 | 319.87 | 956.60 | 957.51 | −0.91 | 0 | 3 | 93 | 10 | K.LLAADAIR.M |
| 698 | 738.83 | 1475.64 | 1474.78 | 0.86 | 0 | 58 | 0.00022 | 1 | R.TSEANNYGTLTK.W |
| Q6UW60 (PCSK4_HUMAN)d | |||||||||
| 319 | 445.28 | 444.27 | 444.23 | 0.04 | 0 | 8 | 1.5 | 3 | K.GPGSK.N |
| 491 | 320.13 | 957.37 | 958.58 | −1.21 | 1 | 13 | 7.9 | 1 | K.TAAPALRV.Q |
| 516 | 337.47 | 1009.39 | 1009.41 | −0.01 | 0 | 17 | 2.9 | 1 | -.MDLPLYAWLSR.C + oxidation (M) |
| P02768 (ALBUMIN_HUMAN)e | |||||||||
| 246 | 712.38 | 711.37 | 711.37 | 0.01 | 0 | (9) | 16 | 2 | K.SEIAHR.F |
| 247 | 356.94 | 711.86 | 711.37 | 0.50 | 0 | 31 | 0.11 | 1 | K.SEVAHR.F |
| 300 | 805.42 | 804.41 | 803.40 | 1.01 | 0 | 3 | 1.1e+002 | 2 | K.ATEDQLK.T |
| 384 | 450.17 | 898.32 | 897.47 | 0.85 | 0 | (23) | 0.74 | 1 | R.LCVLHEK.T |
| 385 | 899.37 | 898.36 | 897.47 | 0.89 | 0 | (16) | 3.8 | 9 | R.LCVLHEK.T |
| 387 | 450.32 | 898.62 | 897.47 | 1.15 | 0 | (17) | 3.2 | 3 | R.LCVLHEK.T |
| 388 | 450.43 | 898.85 | 897.47 | 1.38 | 0 | 27 | 0.37 | 1 | R.LCTVATL.T |
| 501 | 367.10 | 1098.29 | 1099.66 | −1.37 | 1 | 9 | 18 | 9 | K.KQTALAELVK.H |
| 517 | 558.66 | 1115.31 | 1115.65 | −0.34 | 1 | 5 | 43 | 6 | K.LATDLTKINK.E |
| 685 | 1479.61 | 1478.60 | 1478.79 | −0.19 | 0 | (0) | 1.1e+002 | 4 | K.LGEYGFQNAILVR.Y |
| 686 | 1479.68 | 1478.67 | 1478.79 | −0.12 | 0 | (24) | 0.45 | 1 | K.LGEYKFQNALLVR.Y |
| 687 | 1479.77 | 1478.76 | 1478.79 | −0.03 | 0 | (16) | 2.9 | 1 | K.LGEYKFQNALLVR.Y |
| 688 | 740.67 | 1479.32 | 1478.79 | 0.53 | 0 | 70 | 1.1e−005 | 1 | K.LGEYKFQNALLVR.Y |
| 689 | 1480.71 | 1479.70 | 1478.79 | 0.91 | 0 | (29) | 0.16 | 1 | K.LGEYKFQNALLVR.Y |
| 690 | 740.87 | 1479.72 | 1478.79 | 0.93 | 0 | (52) | 0.00077 | 1 | K.LGEYKFQNALLVR.Y |
| 691 | 741.39 | 1480.76 | 1478.79 | 1.97 | 0 | (14) | 5.6 | 1 | K.LGEYKFQNALLVR.Y |
| 823 | 941.90 | 1881.78 | 1881.93 | −0.15 | 0 | 17 | 1.8 | 1 | R.RPCFSALEVDETYVPK.E |
a
Search parameters: Type of search: MS/MS ion search; enzyme: trypsin; fixed modifications: carbamidomethyl (C); variable modifications: oxidation (M); mass values: monoisotopic; protein mass: unrestricted; peptide mass tolerance: ±2 Da; fragment mass tolerance: ±0.8 Da; max missed cleavages: 1; instrument type: ESI-TRAP.
b
P00739 (HPTR_HUMAN), haptoglobin-related protein – human; mass 39020, score 143, query matched 8.
c
P09958 (FURIN_HUMAN), furin protein – human; mass 86778, score 58, query matched 2.
d
Q6UW60 (PCSK4_HUMAN), proprotein convertase subtilisin/kexin type 4; mass 83115, score 38, query matched 3.
e
P02768 (ALBUMIN_HUMAN), albumin; mass 70710, score 157, query matched 18.