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. 2003 Oct 21;32(2):302–308. doi: 10.1016/j.pep.2003.08.016

Table 1.

Purification Scheme of SARS_3CLpro expressed in E. coli transformed with pQE30-SARS_3CLpro (1 L culture)

Step Total protein (mg) SARS_3CLpro (mg) Purification factor 3CLpro yield (%)
Extraction 500 50 1 100
Ni2+-affinity column 50 40 8 80
Gel filtration 15 15 10 30