Fig. 1.

Characterization of the intracellular delivery of proteins and viral particles by PIDR. (A) FITC-conjugated mouse IgG (left panels) or recombinant PE (center panels) was introduced into Huh7OK1 cells by the treatment with PIDR or a lipofection reagent. The expression of GFP upon infection of a pseudotype VSV lacking VSVG (VSV∆Gpv/GFP) into Huh7OK1 cells in the presence (lower panel) and absence (upper panel) of PIDR was examined (right panels). (B) The effect of the presence of neutralization antibody on the delivery of viral particles by PIDR. Expression of GFP upon transduction of a VSVpv/GFP into Huh7OK1 cells in the presence of neutralization antibody and PIDR was examined. (C) The receptor-independent entry of viral particles was confirmed by using VSV∆Gpv/luc and VSVpv/luc carrying the luciferase gene as a reporter.