Fig. 3.

Effect of PIDR on the infection with HCVser. (A) The neutralizing effect of antibodies in the CHS was determined by a neutralization assay using the pseudotype viruses. White and gray bars indicated VSVpv and HCVpv, respectively. (B) The effect of PIDR on the entry of HCVser in the CHS into Huh7OK1 cells. HCV-RNA titers in cells 24 h post-inoculation with HCVser in the presence and absence of PIDR are indicated by gray and white bars, respectively. (C) To determine the internalization of HCVser into Huh7OK1 cells, cells inoculated with the CHS in the presence (gray bar) or absence (white bars) of PIDR were trypsinized and reseeded into a new culture plate at 24 h post-infection, and HCV-RNA titers in the cells were determined at 24 h post-inoculation.