Skip to main content
. 2020 Mar 31;12:2381–2391. doi: 10.2147/CMAR.S236565

Figure 5.

Figure 5

B3GNT3 is a direct target of miR-149-5p. (A) Putative binding sequences of miR-149-5p on the 3ʹ-UTR of B3GNT3 and the mutated sequences of 3ʹ-UTR of B3GNT3. (B) The expression levels of miR-149-5p in lung cancer or control normal tissues were analyzed by qRT-PCR. (C) Pearson correlation analysis of miR-149-5p expression and B3GNT3 expression in lung cancer tissues. (D) Luciferase reporter vector containing WT 3ʹ-UTR of B3GNT3 or mutated 3ʹ-UTR of B3GNT3 were co-transfected with miR-149-5p mimics into HEK293 cells. Relative luciferase activity was analyzed 48 hrs later. (E) A549 or H1299 cells were left untreated (blank), or transfected with miR-149-5p mimics, miR-149-5p inhibitor or relative negative controls. The expression of B3GNT3 was analyzed by qRT-PCR 48 hrs later. (F, G) The expression of B3GNT3 protein in A549 or H1299 cells left untreated (blank), or transfected with miR-149-5p mimics, miR-149-5p inhibitor or relative negative controls was analyzed by Western blot. **p < 0.01.