Table 1.

Comparison of the LacZ marker rescue assay method and real-time PCR method using each canine live vaccine approved in Japan.

Product (Vaccine)	Composed of viral agentsa	Production cell for canine parvovirus	LacZ marker rescue assay method	Real-Time PCR (copy number) per one vial
A	D/Ad2/PI/P	Feline, Fibroblast	−	166,567
B	D/Ad2/PI/P/L	Feline, Fibroblast	−	100,138
C	D/P	Feline, Fibroblast	−	79,166
D	D/Ad2/PI/P	Feline, Kidney	−	299,425
E	D/Ad2/PI/P/C/L	Feline, Kidney	−	219,820
F	D/Ad2/PI/P	Canine, Kidney	−	<100
G	D/Ad2/PI/P/C	Canine, Kidney	−	<100
H	D/Ad2/PI/P/C/L	Canine, Kidney	−	<100
I	D/Ad2/PI/P/L	Feline, Kidney	+	56,133,603
J	D/Ad2/PI/P/C/L	Crandell feline kidney	+	115,608,530
K	D/Ad2/PI/P/C/L	Crandell feline kidney	+	158,325,451
L	D/Ad2/PI/P/C	Crandell feline kidney	+	146,739,904
M	D/Ad2/PI/P/L	Mink, Lung	+	1,352,784,971
N	D/Ad2/P	Mink, Lung	+	395,363,668
O	D/Ad2/PI/P/C	Canine, Kidney	−	<100
P	D/Ad2/PI/P/C/L	Canine, Kidney	NDb	<100
Q	P	Canine, Kidney	NDb	<100
R	D/Ad2/PI/P	Crandell feline kidney	−	17,521,335
S	D/Ad2/PI/P/L	Crandell feline kidney	−	7,526,241
T	P	Crandell feline kidney	−	1,880,984

D; canine distemper virus.

Ad2; canine adenovirus (CAdV-2).

PI; canine parainfluenza virus.

P; canine parvovirus.

C; canine coronavirus.

L; leptospira.

^aCode.

^bNot done because of cytotoxicity.