Table 1.

Oligonucleotide primers and amplification conditions used in this study

Primer name	Sequence (5′–3′)	Purpose	Thermal cycling condition
RBGAPDH1 FW	AGTCGCAAGACAGACTGAGGC	RT-PCR isolation of ORF sequence	30 Cycles of 94 °C for 45 s, 60 °C for 45 s, 72 °C for 1.5 min, with an initial denaturation at 94 °C for 4 min
RBGAPDH1 RV	ATTCCACCACTGACTGCAGTAC
RBGAPDH2 FW	CATCAGCCGTGAGGTAACTCT
RBGAPDH2 RV	AAGCGGAGTTACAGTAGCTCTG
RBGAPDH1-1F	TAAACCAACCAGCCAATCGAC	Real-time RT-PCR assay	40 Cycles of 94 °C for 20 s, 58 °C for 20 s, 72 °C for 30 s, with an initial denaturation at 94 °C for 3 min
RBGAPDH1-1R	CAGACAAAGAGTCTCACACC
RBGAPDH2-1F	GTCTGTCCAAGCCTGCATCT
RBGAPDH2-1R	GTGTAGTAGAGCTAAGGGGT
qRB18S 1F	TACCACATCCAAGGAAGGCA
qRB18S 1R	TTCCTAGCTGCGGTATTCAG
RB18S RV	AGAATTTCACCTCTAGCGGC	Preparation of 18S rRNA template during reverse transcription