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Purification of dengue rNS1 protein from E. coli in 1 L culture.
| Purification steps | Total protein (mg) | rNS1 (mg) | Recovery (%) |
|---|---|---|---|
| Inclusion bodies | 250 | 232 | 92.8 |
| IMAC elutionsa | 20 | 12 | 60 |
| Refolding | 12 | 12 | 100 |
In each batch of IMAC purification, 20 mg of protein was used.
