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Different refolding conditions and yield.
| Protein | GSH:GSSG (mM) | Refolding TA buffer | Protein conc. in refolding buffer (μg/ml) | Yield (%) |
|---|---|---|---|---|
| rNS1 | 1:0.1 | 50 mM Tris–Cl, pH 8.0, 0.4 M l-arginine | 100 | No aggregation (100) |
| rNS1 | 1:0.1 | 50 mM Tris–Cl, pH 8.0, 0.4 M l-arginine | 75 | No aggregation (100) |
| rNS1 | 1:0.1 | 50 mM Tris–Cl, pH 8.0, 0.4 M l-arginine | 50 | No aggregation (100) |
