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. 2005 Feb 23;42(1):100–110. doi: 10.1016/j.pep.2005.02.004

Fig. 5.

Fig. 5

Detection of proteins in samples from various steps of a typical purification of SARS-CoV 3CL protease. Aliquots of the samples (each containing ∼5 μg protein) were separated on a 12% SDS-gel and stained with Coomassie blue. The migration positions of the SUMO-fusion and the proteins resulting from the cleavage are as indicated.