Table 2.
Summary of the SARS-CoV proteins resulting from representative purifications of 1 L E. coli culture
| Proteins | 3CL | Nc | Spk C |
|---|---|---|---|
| Starting samples for purification | Soluble fraction (224 mg) | Soluble fraction (189 mg) | Insoluble fraction (66 mg) |
| Purified SUMO-fusions | 101 mg | 66 mg | 24 mg |
| Purified SARS-CoV proteins | 56 mg | 26 mg | 12 mg |
| Purity | >95% | >95% | ∼30% |
The SARS-CoV proteins fused with SUMO were expressed in E. coli and induced at 20 °C overnight. The wet weights of the cells harvested from 1 L of E. coli culture for the 3CL, Nc, and Spk C were 14, 13, and 10 g, respectively. The samples were prepared and purified as described in Materials and methods.