TABLE 1.

Clinical specimens for validating 23S‐5S assay

Sample type	Cultures positive (+), n	PCR positive (+), n	PCR negative (−), n	Cultures (−)/PCR (+), n	mip sequence analysis of PCR+ but culture− samplesa	Total
Sputum	9	18	40	9	L. pneumophila (n = 5) L. longbeachae (n = 2) L. cincinnatiensis (n = 1) Novel Legionella spp. (n = 1)	58
Lung tissue	24	31	28	7	L. pneumophila (n = 6) Undetermined (n = 1)b	59
Bronchoalveolar lavage	2	3	4	1	L. micdadei (n = 1)	7
Bronchial swab	2	2	0	0	Not applicable	2
Nasopharyngeal swab	2	2	0	0	Not applicable	2
Blood	0	8	0	8	L. pneumophila (n = 2) Novel Legionella spp.(n = 6)	8
Liver	0	0	1	0	Not applicablec	1
Trans‐tracheal aspirate	0	0	1	0	Not applicablec	1
Spleen	0	0	1	0	Not applicablec	1
Pleural fluid	0	1	1	1	L. pneumophila (n = 1)d	2
Formalin‐fixed and paraffin embedded tissue (FFPE)	0	1	7	1	L. pneumophila (n = 1)d	8
Total number of specimens	39e	66	83	27	27	149
Percentage of specimens	26%	44%	56%	18%	18%	100.0%

^amip sequence analysis for all culture−/PCR+ samples unless otherwise indicated.

^bNo sample left for sequencing.

^cNot applicable for sequence analysis.

^dAmplicon sequence analysis only.

^ePositive for Legionella pneumophila.