Table 1.
Ellipticity and stability of the immunogen peptides studied
| Peptide namea | [θ]222b (degrees cm2 dmol−1) | T1/2c (°C) |
|---|---|---|
| HRN1 | −36 500 | >95 (82) |
| HRN2 | −35 100 | >95 (76) |
| HRC1 | −30 000 | >95 (71) |
| HRC2 | −31 200 | >95 (56) |
a
Name of each peptide studied. The sequence of each peptide is shown in Fig. 1.
b
The mean residue molar ellipticity at 222 nm was measured at 22 °C in benign buffer (0.1 M KCl, 0.05 M K2PO4, pH 7).
c
T1/2 is the transition midpoint temperature at which there is a 50% decrease in molar ellipticity [θ]222 compared with the fully folded peptide determined by CD at 5 °C. Values in brackets represent the T1/2 temperature for the reduced form of the peptide.