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. 2003 Sep 24;28(3):233–238. doi: 10.1016/j.jcv.2003.08.004

Table 1.

Detection of SARS CoV in clinical specimens by different conventional and real-time RT-PCR protocols in relation to time after onset of disease

Day of onset Sample Size Number of positives
Conventional RT-PCR assay Conventional RT-PCR assay with a modified RNA extraction protocol* Real-time RT-PCR assay with a modified RNA extraction protocol*, **
1 8 0 (0%) 2 (25%) 5 (63%)
2 16 3 (19%) 8 (50%) 14 (88%)
3 26 8 (31%) 12 (46%) 21 (81%)
*

The overall detection rate of the assay is statistically different from that of the conventional RT-PCR assay (McNemar's test, P<0.001).

**

The overall detection rate of the assay is statistically different from that of the conventional RT-PCR assay with a modified RNA extraction protocol (McNemar's test, P<0.0001).