Figure 5.

ΔPABP1 Inhibits Negative Strand RNA Synthesis In Vitro

(A) Recombinant PABP1-H6 and ΔPABP1-H6 do not effect translation in vitro. Replicon RNA was preincubated with 500 nM of recombinant protein for 5 min and then used to program a translation/replication extract. Luciferase activity was measured at 3 hr.

(B) Recombinant ΔPABP1-H6 inhibits negative strand RNA synthesis. Preinitiation complexes were isolated from the reactions described above, and RNA synthesis was monitored by the incorporation of radiolabel in newly synthesized RNA. Samples were taken after 30, 60, and 90 min, and the RNA was isolated and analyzed on a 1% native agarose gel