Table 2.

Summary of the EMEA guideline [28] on how to interpretate of positive Pestivirus PCR results.

Results of the test	What should be done
PCR is negative	No further tests. Product considered as non-contaminated.
PCR is negative or in vitro test is negative	No further test needed at this stage (unless justified: not optimal conditions for carrying out the in vitro test, see above). Product considered as non-contaminated.
In vitro test is positive	No need for in vivo test. Product considered as contaminated even if the PCR is negative.
PCR+/in vitro test cannot be carried out	The in vivo test is performed (this is done in the case of risk analysis for interference, under the BVD eradication programme) to verify the possibility of a contamination.
PCR+/in vivo test +	Product considered as contaminated.