Fig. 4.

Western blot assay of the recombinant S450-650. Affinity purified S450-650 was run in two identical 12% SDS–PAGE gels with pre-stained protein molecular weight markers (M) in lane 1. One of the gels was stained with Coomassie blue (lane 2), and the other transferred onto cellulose nitrite membrane. WB was performed using mouse anti-His mAb (lane 3), serum from PT31 (lane 4) or PT32 (lane 5) or mice immunized with S450-650 (lane 6) was used as the first Ab. The secondary Ab was HRP-conjugated goat-anti-human IgG (lanes 4 and 5) or goat-anti-mouse IgG (lanes 3 and 6).