Fig. 1.

SEM photograph of D. polymorphus on Ag/Al₂O₃ and Cu/Al₂O₃ wafers treated for 5 min. (a) 10,000× on Ag/Al₂O₃. (b) 20,000× on Ag/Al₂O₃. (c) 3000× on Cu/Al₂O₃. (d) 12,000× on Cu/Al₂O₃. 20 μl of 10⁸ CFU/ml yeast cell was loaded on the surfaces of the wafers at room temperature in air. After 5 min, the wafers were fixed with glutaraldehyde and osmium tetroxide, drained with ethanol/water in increasing concentrations of ethanol. The absolute ethanol was replaced by dimethoxymethane, and the samples underwent critical point drying with CO₂. The wafers were glued onto stages with conductive silver and metallized with gold. The samples were microscoped and photographed with a scanning electron microscope (Fei QUANTA 200).