Table 3.
Results of the sensitivity test for the four-plex RT-qPCR method for the detection of ZIKV and CHIKV.
| 1000 cp | 100 cp | 50 cp | 10 cp | 5 cp | 1 cp | 0.1 cp | NTC | |
|---|---|---|---|---|---|---|---|---|
| CHIKV-a | 28.2–28.2 | 31.9–31.5 | 33.3–32.9 | 35.2–35.9 | 35.7–36.1 | 40.3–ND | ND–ND | ND |
| 28.4–28.5 | 32.2–32.2 | 33.2–33.1 | 35.8–35.7 | 36.8–36.0 | 38.6–ND | ND–ND | ND | |
| CHIKV-b | 30.1–30.0 | 35.0–34.0 | 35.8–36.1 | ND–ND | ND–ND | ND–ND | ND–ND | ND |
| 28.3–28.4 | 31.9–31.5 | 33.0–33.8 | 36.3–ND | ND–ND | ND–ND | ND–ND | ND | |
| ZIKV-a | 31.9–32.6 | 35.1–35.8 | 37.6–36.6 | ND–ND | ND–ND | ND–ND | ND–ND | ND |
| 32.1–32.1 | 34.8–34.5 | 35.7–ND | ND–ND | ND–ND | ND–ND | ND–ND | ND | |
| ZIKV-b | 32.0–33.2 | 36.3–36.9 | 38.4–38.0 | 39.1–39.9 | ND–ND | ND–ND | ND–ND | ND |
| 33.3–33.2 | 37.3–37.4 | 38.2–38.2 | 40.1–ND | ND–ND | ND–ND | ND–ND | ND |
CHIKV, chikungunya virus; ZIKV, Zika virus. Cut-off used = 38. ND: not determined (Cq >45). Results in italic indicate the limit of detection (LOD) of the single run.