Table 4.
Scheme of the samples in which IL-1β was repeatedly determined
| Cat | Sampling number | Batch 2 | Batch 3 | Batch 4 | Batch 5 | Batch 6 | Batch 7 | Batch 8 | Mean | SD | CV |
|---|---|---|---|---|---|---|---|---|---|---|---|
| A (FCoV) | 2 | 68.8 | 58.6 | 65.5 | 64.3 | 5.2 | 8.1 | ||||
| 3 | 85.8 | 89.3 | 87.5 | 2.5 | 2.9 | ||||||
| 4 | 85.2 | 94.6 | 89.9 | 6.7 | 7.4 | ||||||
| 5 | 91.4 | 92.6 | 92.0 | 0.8 | 0.9 | ||||||
| B (FCoV) | 2 | 47.6 | 53.2 | 50.4 | 4.0 | 7.9 | |||||
| 3 | 68.8 | 83.3 | 76.0 | 10.2 | 13.5 | ||||||
| 4 | 80.9 | 72.1 | 76.5 | 6.2 | 8.1 | ||||||
| C (FCoV) | 2 | 76.8 | 79.9 | 78.4 | 2.2 | 2.9 | |||||
| 3 | 39.8 | 55.3 | 47.6 | 11.0 | 23.1 | ||||||
| D (FCoV) | 2 | 46.1 | 54.3 | 50.2 | 5.8 | 11.6 | |||||
| 3 | 74.8 | 77.4 | 76.1 | 1.8 | 2.4 | ||||||
| H (SPF) | 33.4 | 56.2 | 65.0 | 55.2 | 57.3 | 53.4 | 11.8 | 22.2 |
Values are expressed as percentage of the mean intensity of the internal calibrator (500 bp ladder) of each batch. The analysis of the repeatability of the computerised quantification of IL-1β expression was performed by examining mean, standard deviation (SD) and coefficient of variation (CV). None of the samples included in batch 1 were repeatedly analysed so no columns referring to batch 1 are therefore included in this table.