Table 3.

Application of serial lectin affinity chromatography (SLAC) in resolution of the glycoprotein microheterogeneity

Serial no.	Sequence of matrix-immobilized lectin columns used for SLAC	Source of glycoprotein under investigation	Reference
1	Concanavalin A (Con A), pea lectin (PSA), leukoagglutinating phytohemagglutinin and D. stramonium agglutinin (DSA)	Different types of Asn-linked oligosaccharides in mouse lymphoma BS5147 cells	[31], [32]
2	Aleuria aurantia lectin (AAL), Ricinuscommunis agglutinin (RCA)-120-WG003, E4-phytohemagglutinin (PHA-E4)	16 different biantennary complex-type Asn-linked sugar chains	[48]
3	AAL, Con A, PHA-E4, and DSA	Asparagine-linked sugar chains of rat kidney aminopeptidase N and dipeptidylpeptidase IV	[49]
4	Con A and DSA	N-linked oligosaccharides released from normal human esophageal epithelium and esophageal squamous carcinoma	[12]
5	Con A, S. nigra agglutinin (SNA), and P. vulgaris leukoagglutinin (LPHA)	N-glycosylation site mapping of human serotransferrin	[50]
6	Con A, PHA-E and jacalin lectin	Sugar-chain heterogeneity of human urinary chorionic gonadotropin (hCG)	[51]
7	Con A, PHA-E, PSA and wheat germ agglutinin (WGA)	Sugar-chain structures of gamma-glutamyltransferase in human renal cell carcinoma	[52]
8	Con A, WGA	Asn-linked sugar-chain structure of prostatic acid phosphatase (differences between human prostate carcinoma and benign prostatic hyperplasia)	[53]
9	Con A, WGA	Asn-linked sugar-chain structures of N-acetyl beta-d-hexosaminidase A (Hex A) (in human renal oncogenesis)	[54]
10	Con A, PHA-E4 and PHA-L4	Asn-linked sugar-chain structures of prostate-specific antigen (PSA)	[55]