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. 2020 Feb 11;2(1):fcaa010. doi: 10.1093/braincomms/fcaa010

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© The Author(s) (2020). Published by Oxford University Press on behalf of the Guarantors of Brain.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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IHC confirmation of synucleinopathy in DLB frontal cortical samples. (A) IHC of the formalin-fixed striatum of MGH DLB brain #1594 with αSyn monoclonal antibody 2F12 demonstrates the striking local heterogeneity of cytopathology. Yellow box: area of high density of Lewy cytopathology; blue box: immediately adjacent area devoid of IHC-detectable αSyn aggregates. Scale bars: 300 μm. (B) Small frozen pieces of tissue were excised from locations adjacent (within ∼5 mm) to the frozen pieces we used for our biochemical extractions. The resultant cryostat sections were stained with αSyn monoclonal antibody 2F12 and nuclei counterstained with haematoxylin. Representative images from two control and two DLB brains show ice crystals resulting from variable freezing of the unfixed tissue. Arrows exemplify LBs; arrowheads exemplify Lewy neurites. Scale bars: 80 μm.