. 2015 Jul 26;33(51):7370–7374. doi: 10.1016/j.vaccine.2015.07.043

Table 2.

Safety test of K40/09 HP50 compared with the K40/09 parent and K40/09 HP40 in 1-day-old SPF chicks.

Administration method^a	Treatment	Mortality (%)	Mean ciliostasis scores^b	Histopathologic lesion scores^c
				Upper trachea	Middle trachea	Lower trachea	Kidney
Control	PBS	0/10 (0)	0.1	0	0	0	0.3

Intraocularly	K40/09 (Parent)	4/10 (40)	2.6^***	1.3^*	1.5^*	1.2^*	2.8^***
	K40/09 HP40	0/10 (0)	1.6	0.4	0.4	0.2	2.9^***
	K40/09 HP50	0/10 (0)	1.2	0.2	0.3	0.1	0.9

Fine spray	K40/09 (Parent)	4/10 (40)	3.3^†††	1.9^†	2.6^†††	1.9^†	2.8^†††
	K40/09 HP40	0/10 (0)	3.2^†††	2^†	1.7^†	1.2	2.2^†††
	K40/09 HP50	0/10 (0)	1.8	0.9	0.7	0.9	0.5

One-day-old SPF chicks were inoculated with IBV (10^4.0 EID₅₀/bird) by eyedrop or fine spray (droplet size = 50 μm).

On day 5 post-vaccination, tracheas were removed from five chickens in each group. Ten trachea rings per chick were prepared (three upper, four middle, and three lower). The rings were examined under low-power magnification and ciliary activity was scored as follows: 0, no ciliostasis; 1, 25% ciliostasis; 2, 50% ciliostasis; 3, 75% ciliostasis; 4, 100% ciliostasis.

Histopathology of the trachea and kidney at 5 days post-vaccination with IBV is reported as histopathologic lesion scores. 0, normal; 1, extensively focal lesions, 2, multifocal lesions; 3, diffuse lesions.

p < 0.05, by two-tailed t-test, compared to the K40/09 HP50 intraocularly vaccinated group.

^***

p < 0.001, by two-tailed t-test, compared to the K40/09 HP50 intraocularly vaccinated group.

^†

p < 0.05, by two-tailed t-test, compared to the K40/09 HP50 vaccinated group by fine spray method.

^†††

p < 0.001, by two-tailed t-test, compared to the K40/09 HP50 vaccinated group by fine spray method.