Table 1.

Sequence, position and specificity of the oligonucleotides used in the study

Assay	Primer/probe	Sequence 5′–3′	Polarity	Specificity	Position	Amplicon size
Type 2/variants MGB probe assaya	CPV2/v-For	GCAGTTAACGGAAACATGGCTTTAG	+	All types	3057–3081d; 772–796e	68 bp
	CPV2/v-Rev	TCAACCAATGACCAAGGTGTTACAA	−		3100–3124d; 815–839e
	CPV2-Pb	FAM-TGTGCATGAATATCAT-MGB	+	Type 2 (old type)	3082–3097d
	CPVv-Pb	VIC-TTTGTGCATGAGTATCAT-MGB	+	Field strains	797–814e
SAH/field assayb	SAH/f-For	CAACAAGATAAAAGACGTGGTGTAACTC	+	All types	3711–3738d; 1426–1453f	85 bp
	SAH/f-Rev	CAACCTCAGCTGGTCTCATAATAGT	−		3771–3795d; 1486–1510f
	SAH-Pb	VIC-AAATGGGAAAAACAAACT-MGB	+	Strain SAH	1454–1471f
	CPVf1-Pb	FAM-AAATGGGAAATACAAACT-MGB	+	Field strains	1454–1471f
39/field assayb	CPV39/f-For	GCATTGGGCTTACCACCATTTCTAA	+	All types	3636–3660d; 1351–1375f	95 bp
	CPV39/f-Rev	CCACGTCTTTTATCTTGTTGAACTCCTATA	−		3701–3730d; 1416–1445f
	CPV39-Pb	VIC-CTTTGCCTCAATCTGAA-MGB	+	Strain CPV-39	1379–1395f
	CPVf2-Pb	FAM-TTTGCCTCAAGCTGAA-MGB	+	Field strains	1380–1395f
Type 2a/2b assayc	CPVa/b-For	AGGAAGATATCCAGAAGGAGATTGGA	+	All types	1719–1744e,f	93 bp
	CPVa/b-Rev	CCAATTGGATCTGTTGGTAGCAATACA	−		1785–1811e,f
	CPVa-Pb	VIC-CTTCCTGTAACAAATGATA-MGB	+	Type 2a	1765–1783e
	CPVb1-Pb	FAM-CTTCCTGTAACAGATGATA-MGB	+	Type 2b	1765–1783f
Type 2b/2c assayc	CPVb/c-For	GAAGATATCCAGAAGGAGATTGGATTCA	+	All types	1721–1748f; 1155–1182g	150 bp
	CPVb/c-Rev	ATGCAGTTAAAGGACCATAAGTATTAAATATATTAGTATAGTTAATTC	−		1823–1870f; 1257–1304g
	CPVb2-Pb	FAM-CCTGTAACAGATGATAAT-MGB	+	Type 2b	1768–1785f
	CPVc-Pb	VIC-CCTGTAACAGAAGATAAT-MGB	+	Type 2c	1202–1219g

^a[17].

^b[18].

^c[11].

^dOligonucleotide positions are referred to the sequence of CPV-2 (old type) strain CPV-b (accession no. M38245).

^eOligonucleotide positions are referred to the sequence of CPV-2a strain CPV-15 (accession no. M24003).

^fOligonucleotide positions are referred to the sequences of CPV-2b strain CPV-39 (accession no. M74849).

^gOligonucleotide positions are referred to the sequences of CPV-2c strain 56/00 (accession no. AY380577).