Table 6.
Fluid accumulation and lesions in calf intestinal loops inoculated with TcdA, TcdB, or both
| Location | Injected with | Fluid accumulation (control/toxin)a | Lesionsb |
|---|---|---|---|
| Duodenum | TcdA | 1 | PMN infiltration, edema, lost epithelium, hemorrhage |
| TcdB | 1 | PMN infiltration, ulceration, necrosis | |
| TcdA/B | 1 | Villous degeneration | |
| Jejunum | TcdA | 0.5 | Villous degeneration, hemorrhage, fibrin accumulation |
| TcdB | 0.21 | PMN infiltration, hemorrhage, necrosis, edema | |
| TcdA/B | 1 | Distended lacteals, lymphocyte infiltration | |
| Ileum | TcdA | 0.8 | PMN infiltration, necrosis |
| TcdB | 1 | Ulcerated villi, edema | |
| TcdA/B | 0.63 | Villous necrosis, edema, necrosis, hemorrhage, inflammatory cell infiltration | |
| Proximal colon | TcdA | 0.8 | PMN infiltration, necrosis, hemorrhage |
| TcdB | 0.13 | PMN infiltration, lymphocyte infiltration into lacteals | |
| TcdA/B | 0.81 | Hemorrhage, erosion, mucus, cellular debris in lumen | |
| Mid-colon | TcdA | 0.53 | PMN infiltration, necrosis, mucus, fibrin in lumen |
| TcdB | 0.8 | PMN infiltration, ulceration | |
| TcdA/B | 0.83 | Villous degeneration, inflammatory cell infiltration, mucus, cellular debris in lumen |
a
Ratio of weight:length of control loop vs. weight:length of principle loop; lower numbers indicate greater toxin effect.
b
Lesions were detected in principal loops only.