Table 1.
Identification of cattle used as inoculums and dogs (2–3 month old) infected by different bovine tissues and Neospora caninum or Hammondia heydorni oocysts shedding.
| Inocula | Bovine no. | Dog no. | Shedding (d.a.i.) | Total number of oocysts | PCR and sequencing analysis |
|---|---|---|---|---|---|
| Brain | B1 | 2 | N | 0 | ND |
| B2 | 6 | 8 | 218 | N. caninum | |
| B3 | 11 | 10, 11 | 1495 | N. caninum | |
| B4 | 18 | 7, 8, 9 | 1165 | N. caninum | |
| Masseter | B1 | 1 | N | 0 | ND |
| B2 + B3 | 7 | 8, 9, 10, 12 | ND | H. heydorni | |
| B2 + B3 | 12 | 6, 9, 10, 11, 12 | ND | H. heydorni | |
| B4 | 16 | 9, 10, 14 | 993 | N. caninum | |
| B4 | 17 | 8, 9, 10 | 1311 | N. caninum | |
| Liver | B1 | 3 | N | 0 | ND |
| B4 | 21 | N | 0 | ND | |
| B4 | 22 | 14 | 317 | N. caninum | |
| Heart | B1 | 5 | 12, 13, 15, 16, 17 | 5100 | N. caninum |
| B2 + B3 | 9 | 6, 7, 8, 10, 11, 12, 13, 14 | ND | H. heydorni | |
| B2 + B3 | 14 | N | 0 | ND | |
| B4 | 19 | 8, 9, 12, 13, 14 | ND | H. heydorni | |
| B4 | 20 | 14 | 309 | N. caninum | |
| Control | 4 | N | 0 | ND | |
| 10 | N | 0 | ND | ||
| 15 | N | 0 | ND | ||
N = negative; ND = not done; d.a.i. = day after infection.