Figure 2.

The conditioned medium (CM) of cancer‐associated fibroblasts (CAFs) enhanced the migration ability of esophageal squamous cell carcinoma cell lines. A, Schematic representation of the in‐direct coculture system. B, Cells cocultured with CAFs gained enhanced migration ability than that in control groups and normal fibroblasts (NFs) groups. C, The effect of CM of CAFs on the migration ability of TE‐1 by using Transwell migration assay. D, Cell migration ability was measured by a wound‐healing assay. The migration distances of the TE‐1 cells cultured with CAFs‐CM were increased. E, The effects of the CM of CAFs on the migration ability of EC109 cells in the Transwell assay. F, The effects of the CM of CAFs on the migration ability of EC109 were measured in the six paired stromal cells using Transwell migration assay. Results shown are representative of three independent experiments. Statistical significance *P < .05, **P < .01 and ***P < .001