Figure 3.

RP11‐81H3.2 directly interacts with miR‐339. A, Bioinformatics analysis predicted the potential RP11‐81H3.2 binding sites of miR‐339. B, HEK293 cells were transfected luciferase reporter vector containing lncRNA RP11‐81H3.2 WT or mutant sequences, together with miR‐NC control, miR‐339 mimics, or miR‐339 inhibitor. Relative luciferase activity was examined using a due‐luciferase reporter kit. C, SGC‐7901 and BGC‐823 GC cells were transfected with sh‐NC or sh‐RP11‐8H3.2, miR‐339 expression was analyzed by qPCR. D, SGC‐7901 and BGC‐823 GC cells were transfected with miR‐NC or miR‐339 mimics, lncRNA RP11‐81H3.2 expression was analyzed by qPCR. E, The expression levels of miR‐339 in GC tissues and adjacent normal tissues were analyzed by qPCR. *P < .05, compared with miR‐NC or adjacent normal tissues; **P＜.01, compared with sh‐NC or miR‐NC