Figure 5.

miR‐339 regulates the HNRNPA1 expression by targeting HNRNPA1 3’‐UTR. A, Bioinformatics analysis indicted the putative complementary binding sites between HNRNPA1 and miR‐339. B, HEK293 cells were co‐transfected with miR‐339 mimics or miR‐NC control and luciferase reporter vectors containing WT 3’‐UTR of HNRNPA1 or mutated 3’‐UTR of HNRNPA1. Relative luciferase activity was analyzed 48 h later. C‐D, GC cell line SGC‐7901 or BGC‐823 was transfected with miR‐NC, miR‐339 mimics or left untreated. HNRNPA1 protein expression was analyzed 48 h later. The representative western blot data were shown and the experiments were repeated at least three times independently. *P < .05, compared with miR‐NC; **P ＜.01, compared with the control