Figure 6.

RP11‐81H3.2‐miR‐339‐HNRNPA1 interaction network regulates the GC cell proliferation, migration, and invasion. SGC‐7901 or BGC‐823 cells were transfected with RP11‐81H3.2 knockdown vector (sh‐RP11‐81H3.2), sh‐RP11‐81H3.2 + miR‐339 inhibitor, sh‐RP11‐81H3.2 + pcDNA3.1‐HNRNPA1, or NC. A, B, The cell proliferation of SGC‐7901 or BGC‐823 cells was assessed by CCK‐8 assay at indicated time points. C, D, The cell invasion capability of SGC‐7901 and BGC‐823 cells was analyzed by transwell assay. E, F, The cell migration capability of SGC‐7901 and BGC‐823 was analyzed by wound healing assay. G, H, cells were stained with Annexin V/PI and cell apoptosis was analyzed by flow cytometry; I, J, HNRNPA1 protein expression was analyzed 48 h later. The representative western blot data were shown and the experiments were repeated at least three times independently. **P < .01 vs NC group, ^## P < .01 vs sh‐RP11‐81H3.2 group